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Part:BBa_K779503:Design

Designed by: Kristjan Eerik Kaseniit   Group: iGEM12_MIT   (2012-09-29)

Long input strand MammoBlock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

3' phosphate added to protect from some 3' exonucleases (suggested by IDT). 5' IR fluorophore added for quantifying oligo levels.

Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. The latter was achieved by using a high G/C content (constrained to be less than 70% by [http://www.sciencemag.org/content/332/6034/1196.abstract Qian et al. 2011]).

See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki].

Source

De novo design.

References