Other
Part:BBa_K779503:Design
Designed by: Kristjan Eerik Kaseniit Group: iGEM12_MIT (2012-09-29)
Long input strand MammoBlock
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
3' phosphate added to protect from some 3' exonucleases (suggested by IDT). 5' IR fluorophore added for quantifying oligo levels.
Sequence designed to be orthogonal to the HEK293 transcriptome, and to have a high melting temperature. The latter was achieved by using a high G/C content (constrained to be less than 70% by [http://www.sciencemag.org/content/332/6034/1196.abstract Qian et al. 2011]).
See more details on design principles with respect to orthogonality at the [http://2012.igem.org/Team:MIT/ResultsSensing#sensing1bio MIT iGEM wiki].
Source
De novo design.